Question: Hi, I had protected sex on 25/12/2013. But during intercourse (which lasted for only 30 seconds or less) i lost erection and withdrawned my penis, but then i realised that condom was slipped during those 30 seconds. On 12th day after the incident I did HIV Duo including p24 antigen (4th Gen) and HIV-1 RNA Qualitative by Real Time PCR. The Duo test result came back negative the very same day. And RNA PCR came back as Not Detected after 4 days. The doctor told the detection limit was 20 copies/ml and is very accurate. The details of that test are given below. My Query is "Can i consider my 12 day RNA PCR test as conclusive. And how much % accurate it is at 12th day" HIV-1 RNA Qualitative by Real Time PCR - Not Detected Clinical Background: The qualitative HIV RNA tests use a process called reverse transcription polymerase chain reaction (RT-PCR). This type of qualitative test reports whether HIV is present in the bloodstream or not. The result is reported as either "detected" or "not detected.” If a qualitative RNA test is positive (detected), then it is confirmed that the patient if infected with HIV. The "qualitative" test is more accurate than the "quantitative" test because qualitative tests are able to detect very low levels of the virus. If a patient has a quantitative test that shows no detectable virus, but has a positive result with the qualitative test, it means there is a very low level of virus that can be detected only with the qualitative test. Note: * This test is performed in CAP/ISO 15189:2007 accredited collaborating laboratory.

Dear Doctor, Many websites are giving totally different window periods for different tests. Some say only by 28th day the 4th Gen Duo test will be conclusive and some say by 6 weeks. Same is the case of RNA PCR test also. Some say 16 days, some say 7 days...Please calrify..Doctor, please note that during the incident my penis was inside her uncovered for few seconds. So can i consider my results on 12th day as conclusive.