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Is HIV Transmission Possible Through Contact With Dried Blood Containing Live HIV?
Question: Hello ! Sorry about disturbing you !!
I was reading literature about how long HIV survive outside body !
And I have question about that !
In experiments is said that HIV in dry blood sample could live for long time in room temperature for 7 days
Dried and held at room temperature, HTLV-III/LAV retains infectivity for more than three days with a reduction of approximately 1 log10TCID50 per nine hours
http://www.ncbi.nlm.nih.gov/pubmed/0000
And in other literature it is said that it cold live stable in dry room temperature rather long time
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC263082/pdf/jcm00002-0311.pdf
Is is said looking in literature CDC
HIV does not survive long outside the human body (such as on surfaces), and it cannot reproduce outside a human host.
http://www.cdc.gov/hiv/basics/transmission.html
As it was discussed also by CDC – that in these experiments HIV viral load is very high
http://answers.webmd.com/answers/0000/can-i-get-hiv-from-anal-sex
And as looking in HIV viral load chart – such high viral load as used in experiment could be in HIV early stage and late stage as 1000 000 per ml
http://www.healthhype.com/cd4-count-dropping-viral-load-stable-in-hiv-infection-graph.html
1.So in conclusion if you get huge amount of dry blood or other body fluid with very high viral load contact like in early stage or late stage in room temperature and – your skin is brooked up you could get HIV infection.
Is it so ?
2.And how much is viral load drop by 1 log10TCID50 in numbers as it is said in one experiment?
4. And I could not find new publications about HIV surviving outside body like 2010 – 2016 it is because the results would be the same, and there is no new experiments.
Thank you !
Good luck !
I was reading literature about how long HIV survive outside body !
And I have question about that !
In experiments is said that HIV in dry blood sample could live for long time in room temperature for 7 days
Dried and held at room temperature, HTLV-III/LAV retains infectivity for more than three days with a reduction of approximately 1 log10TCID50 per nine hours
http://www.ncbi.nlm.nih.gov/pubmed/0000
And in other literature it is said that it cold live stable in dry room temperature rather long time
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC263082/pdf/jcm00002-0311.pdf
Is is said looking in literature CDC
HIV does not survive long outside the human body (such as on surfaces), and it cannot reproduce outside a human host.
http://www.cdc.gov/hiv/basics/transmission.html
As it was discussed also by CDC – that in these experiments HIV viral load is very high
http://answers.webmd.com/answers/0000/can-i-get-hiv-from-anal-sex
And as looking in HIV viral load chart – such high viral load as used in experiment could be in HIV early stage and late stage as 1000 000 per ml
http://www.healthhype.com/cd4-count-dropping-viral-load-stable-in-hiv-infection-graph.html
1.So in conclusion if you get huge amount of dry blood or other body fluid with very high viral load contact like in early stage or late stage in room temperature and – your skin is brooked up you could get HIV infection.
Is it so ?
2.And how much is viral load drop by 1 log10TCID50 in numbers as it is said in one experiment?
4. And I could not find new publications about HIV surviving outside body like 2010 – 2016 it is because the results would be the same, and there is no new experiments.
Thank you !
Good luck !
Brief Answer:
Kindly elaborate your concern related to HIV.
Detailed Answer:
Hello,
Welcome and thanks for posting your query to the forum.
I can understand your concern for HIV and it's infectivity with various related concerns.
I have gone through your complete query and noted your concern. I can make out that you ha e had some kind of exposure related to HIV or an unprotected contact because of which you are searching for infectivity and things related to HIV virus.
In order to help you best I humbly request you to kindly provide a detailed history of your type of exposure and all the points or your apprehensions related to same. Do give details of any of the important points which are worth to be noted to help you best regarding your issue.
With regards,
Dr Sanjay Kanodia
MD Dermatology & Sexually transmitted diseases
Kindly elaborate your concern related to HIV.
Detailed Answer:
Hello,
Welcome and thanks for posting your query to the forum.
I can understand your concern for HIV and it's infectivity with various related concerns.
I have gone through your complete query and noted your concern. I can make out that you ha e had some kind of exposure related to HIV or an unprotected contact because of which you are searching for infectivity and things related to HIV virus.
In order to help you best I humbly request you to kindly provide a detailed history of your type of exposure and all the points or your apprehensions related to same. Do give details of any of the important points which are worth to be noted to help you best regarding your issue.
With regards,
Dr Sanjay Kanodia
MD Dermatology & Sexually transmitted diseases
Above answer was peer-reviewed by :
Dr. Chakravarthy Mazumdar
Of course you know that man first think with his instincts and then with his brain.
I was visiting erotic massage as it suppose to be very safe practice to avoid HIV transmission. And it is said in all literature. But I m thinking person , so I was reading literature and putting things together .
So as I said I hade erotic massage as man who wanted to get relax - and I choose it as it is the most safes intimate contact. So I had erotic massage contact with the sex commercial worker, and there was only her hands and breasts contact to my body and my penis – nothing more. After erotic massage I start to thin. As it is said that HIV virus is weak outside the body, and once it is dry it is inactive. And I was looking in literature how really long HIV survive outside body! As I was thinking if before me there was clients who practice the same erotic massage – so it is possible that there was sperm of erotic massage lady body, hands and breasts. And if she practice massage on my body and even potential body fluid was dry it still could be active and infective. And she did no use disinfection to clean her body. So I look in literature.
1. Is is said looking in literature CDC
HIV does not survive long outside the human body (such as on surfaces), and it cannot reproduce outside a human host. It is not spread by
http://www.cdc.gov/hiv/basics/transmission.html
2. I other literature
In certain conditions, the virus can survive outside the body for several weeks.
http://www.aidsmap.com/Survival-outside-the-body/page/0000/
Drying the virus on a glass surface or freezing caused a 5-12 fold and 4-5 fold decrease of activity, respectively
http://www.ncbi.nlm.nih.gov/pubmed/0000
And this article full text
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC263082/
3. But in other article it is said that it could die in high temperature but in room temperature it could be rather stable and infective
Under our experimental conditions utilizing a highly concentrated viral preparation, virus with an initial infectious titer of approximately 7 log10 tissue culture infectious dose (TCID50) per milliliter can be recovered for more than a week from an aqueous environment held at room temperature (23 to 27 degrees C) or at 36 to 37 degrees C. Virus recovery is reduced at a rate of approximately 1 log10TCID50 per 20 minutes when held at 54 to 56 degrees C. Dried and held at room temperature, HTLV-III/LAV retains infectivity for more than three days with a reduction of approximately 1 log10TCID50 per nine hours
http://www.ncbi.nlm.nih.gov/pubmed/0000
I was looking for new publication but I didn’t find any new study – I don’t know why, I was looking also un pub med – but I didn’t find new publication.
So in conclusion
If erotic massage therapist had sperm on her body in dry form and if it could have high viral load as in early infection stage – and girl made my penis massage with her breasts and hands I m at risk of hiv infection ?
I think risk is rather low – as also massage oil change pH
In sperm HIV viral load is lower
And other factors
Could you please give me you comment !
Good luck for you !
I was visiting erotic massage as it suppose to be very safe practice to avoid HIV transmission. And it is said in all literature. But I m thinking person , so I was reading literature and putting things together .
So as I said I hade erotic massage as man who wanted to get relax - and I choose it as it is the most safes intimate contact. So I had erotic massage contact with the sex commercial worker, and there was only her hands and breasts contact to my body and my penis – nothing more. After erotic massage I start to thin. As it is said that HIV virus is weak outside the body, and once it is dry it is inactive. And I was looking in literature how really long HIV survive outside body! As I was thinking if before me there was clients who practice the same erotic massage – so it is possible that there was sperm of erotic massage lady body, hands and breasts. And if she practice massage on my body and even potential body fluid was dry it still could be active and infective. And she did no use disinfection to clean her body. So I look in literature.
1. Is is said looking in literature CDC
HIV does not survive long outside the human body (such as on surfaces), and it cannot reproduce outside a human host. It is not spread by
http://www.cdc.gov/hiv/basics/transmission.html
2. I other literature
In certain conditions, the virus can survive outside the body for several weeks.
http://www.aidsmap.com/Survival-outside-the-body/page/0000/
Drying the virus on a glass surface or freezing caused a 5-12 fold and 4-5 fold decrease of activity, respectively
http://www.ncbi.nlm.nih.gov/pubmed/0000
And this article full text
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC263082/
3. But in other article it is said that it could die in high temperature but in room temperature it could be rather stable and infective
Under our experimental conditions utilizing a highly concentrated viral preparation, virus with an initial infectious titer of approximately 7 log10 tissue culture infectious dose (TCID50) per milliliter can be recovered for more than a week from an aqueous environment held at room temperature (23 to 27 degrees C) or at 36 to 37 degrees C. Virus recovery is reduced at a rate of approximately 1 log10TCID50 per 20 minutes when held at 54 to 56 degrees C. Dried and held at room temperature, HTLV-III/LAV retains infectivity for more than three days with a reduction of approximately 1 log10TCID50 per nine hours
http://www.ncbi.nlm.nih.gov/pubmed/0000
I was looking for new publication but I didn’t find any new study – I don’t know why, I was looking also un pub med – but I didn’t find new publication.
So in conclusion
If erotic massage therapist had sperm on her body in dry form and if it could have high viral load as in early infection stage – and girl made my penis massage with her breasts and hands I m at risk of hiv infection ?
I think risk is rather low – as also massage oil change pH
In sperm HIV viral load is lower
And other factors
Could you please give me you comment !
Good luck for you !
Brief Answer:
Clinically no chance of HIV infection.
Detailed Answer:
Hello young fellow,
Welcome back and thanks a lot for your detailed information.
I have completely analyzed your query and noted all your concerns.
I appreciate your part of research done and all the information shared in context of any possibility of HIV.
First of all let me reassure on the part of my experience as a clinical expert that I very often counsel your kind of cases with similar episodes and getting anxious regarding any possibility of HIV. The good news till now is that I have never found any positive case till now.
It is basically on the grounds of the clinical fact that HIV virus requires an entry point through cut or wound by which it can enter into the body. Moreover there is an infective dose of virus that is required to cause the infection. Massage is not at all mode of infection by any means. Yes the virus definitely gets inactive in contact with air.
The live virus found in dried blood or any other material is basically meant for the basis of laboratory purpose.
There is difference in what is observed in the laboratory and what is experienced clinically. In laboratories it is found that hiv virus is present in saliva but the same is never considered to be mode of infection by kissing.
So as a clinician I reassure you fully that there are practically no chance of HIV infection in your tyoe of exposure.
I hope above information helps.
With regards ,
Dr Sanjay Kanodia
MD Dermatology & Sexually transmitted diseases
Clinically no chance of HIV infection.
Detailed Answer:
Hello young fellow,
Welcome back and thanks a lot for your detailed information.
I have completely analyzed your query and noted all your concerns.
I appreciate your part of research done and all the information shared in context of any possibility of HIV.
First of all let me reassure on the part of my experience as a clinical expert that I very often counsel your kind of cases with similar episodes and getting anxious regarding any possibility of HIV. The good news till now is that I have never found any positive case till now.
It is basically on the grounds of the clinical fact that HIV virus requires an entry point through cut or wound by which it can enter into the body. Moreover there is an infective dose of virus that is required to cause the infection. Massage is not at all mode of infection by any means. Yes the virus definitely gets inactive in contact with air.
The live virus found in dried blood or any other material is basically meant for the basis of laboratory purpose.
There is difference in what is observed in the laboratory and what is experienced clinically. In laboratories it is found that hiv virus is present in saliva but the same is never considered to be mode of infection by kissing.
So as a clinician I reassure you fully that there are practically no chance of HIV infection in your tyoe of exposure.
I hope above information helps.
With regards ,
Dr Sanjay Kanodia
MD Dermatology & Sexually transmitted diseases
Above answer was peer-reviewed by :
Dr. Prasad
Thank you
For your help – Just one short question about HIV – how much particles is needed to infect some on !
I was doing research for better understanding – And there was material.
The 50% infective dose of HIV in blood exposures ranges from one virion (two copies RNA) in primary infection with CCR5 co-receptor using strains of HIV-1 to 65,000 copies HIV-1 RNA in blood from an asymptomatic source patient. The median transmission risks for unsafe intravenous or intramuscular injections using equipment cleaned but not sterilized after use on a symptomatic pre-AIDS patient are 1.8% (95% confidence interval [CI] 0.1-3.2%) and 0.8% (95% CI 0.1-1.4%), respectively
http://www.ncbi.nlm.nih.gov/pubmed/0000
And here is full text explanation
http://www.who.int/injection_safety/toolbox/2009_Review-on-infective-dose-for-HIV-infection.pdf
So as I understand there should be 4 copies (as it would be 100 %) of HIV RNA to transmit HIV to someone. But as I understand the dose is lover because it is infected in blood stream.
Did I correctly understand it ?
Thank you very much for your help !
For your help – Just one short question about HIV – how much particles is needed to infect some on !
I was doing research for better understanding – And there was material.
The 50% infective dose of HIV in blood exposures ranges from one virion (two copies RNA) in primary infection with CCR5 co-receptor using strains of HIV-1 to 65,000 copies HIV-1 RNA in blood from an asymptomatic source patient. The median transmission risks for unsafe intravenous or intramuscular injections using equipment cleaned but not sterilized after use on a symptomatic pre-AIDS patient are 1.8% (95% confidence interval [CI] 0.1-3.2%) and 0.8% (95% CI 0.1-1.4%), respectively
http://www.ncbi.nlm.nih.gov/pubmed/0000
And here is full text explanation
http://www.who.int/injection_safety/toolbox/2009_Review-on-infective-dose-for-HIV-infection.pdf
So as I understand there should be 4 copies (as it would be 100 %) of HIV RNA to transmit HIV to someone. But as I understand the dose is lover because it is infected in blood stream.
Did I correctly understand it ?
Thank you very much for your help !
Brief Answer:
You are pretty correct in your understanding. Relax
Detailed Answer:
Dear young fellow,
Welcome back and thanks a lot for your so swift follow up.
I am astonished to see your part of details and research done !!!!
I don't find this kind of attitude even in my medical students.
You are very much correct in your understanding that it is basically by direct blood borne or needle injury going directly into the blood where the infective dose could be such low.
In saliva even if millions or trillions of copies are present then also the infection is not possible. The same is true for body massage getting even more safer than any of mode of infection.
My all best wishes to you for your healthy life ahead.
With regards,
Dr Sanjay Kanodia
MD Dermatology & Sexually transmitted diseases
You are pretty correct in your understanding. Relax
Detailed Answer:
Dear young fellow,
Welcome back and thanks a lot for your so swift follow up.
I am astonished to see your part of details and research done !!!!
I don't find this kind of attitude even in my medical students.
You are very much correct in your understanding that it is basically by direct blood borne or needle injury going directly into the blood where the infective dose could be such low.
In saliva even if millions or trillions of copies are present then also the infection is not possible. The same is true for body massage getting even more safer than any of mode of infection.
My all best wishes to you for your healthy life ahead.
With regards,
Dr Sanjay Kanodia
MD Dermatology & Sexually transmitted diseases
Above answer was peer-reviewed by :
Dr. Arnab Banerjee
Thank you very much !
For short understanding juts answer yes below my question
1. so once even 4 RNA HIV can transmitted when injected in blood stream
2. For other activities like sex, bloody fight should be much more HIV RNA viral particles like 100 ? Could it be so ?
3. And there is very low risk of touching broken skin with may own hand that there could be blood particles of hiv like in summer when there is lot of mosquito’s from other people around me ? because even broken skin will have more protecting immunity mechanism .
Brief Answer:
Hiv transmission : skin to skin contact is not a mode
Detailed Answer:
Hello,
Welcome back and thanks for your further follow up.
You are correct in your understanding that for direct blood transmission even minimum of virus could cause hiv. For rest like sexual contact require even higher number of viruses.
In your queries the various means of transmission are basically laboratory models for experimental purpose to see the transmission. For all practical purpose or clinical purpose the scenario is variable from one condition to another. Literature is having innumerous articles and reports of various models but the most important part is the practical clinical consideration.
Let you understand a basic fact that HIV never spreads by intact skin to skin contact. Massage is never a mode of HIV transmission. In literature you will find so many of reports of transmission by blood transmission but it it so always when there is direct blood to blood contact.
Mosquito by in addition never leads to hiv transmission.
So now relax yourself and do not worry about any possibility of HIV in uour case.
With best regards,
Dr Sanjay Kanodia
MD Dermatology & Sexually transmitted diseases
Hiv transmission : skin to skin contact is not a mode
Detailed Answer:
Hello,
Welcome back and thanks for your further follow up.
You are correct in your understanding that for direct blood transmission even minimum of virus could cause hiv. For rest like sexual contact require even higher number of viruses.
In your queries the various means of transmission are basically laboratory models for experimental purpose to see the transmission. For all practical purpose or clinical purpose the scenario is variable from one condition to another. Literature is having innumerous articles and reports of various models but the most important part is the practical clinical consideration.
Let you understand a basic fact that HIV never spreads by intact skin to skin contact. Massage is never a mode of HIV transmission. In literature you will find so many of reports of transmission by blood transmission but it it so always when there is direct blood to blood contact.
Mosquito by in addition never leads to hiv transmission.
So now relax yourself and do not worry about any possibility of HIV in uour case.
With best regards,
Dr Sanjay Kanodia
MD Dermatology & Sexually transmitted diseases
Above answer was peer-reviewed by :
Dr. Raju A.T
Hello !
Thank you very much !
The short answers would be great – I will understand in shortly
1, i know it can not be transmitted by intact skin - the question was different
And there is very low risk of touching broken skin with my own hand that there could be blood particles of hiv like in summer when there is lot of mosquito’s from other people around me ? because even broken skin will have more protecting immunity mechanism.
but i understand that risk is rather low - is it ?
2. Second question. The viral drop of 1 log10TCID50 per nine hours would be in 100 % as 2 log10 ? is it so ? as it said
HTLV-III/LAV retains infectivity for more than three days with a reduction of approximately 1 log10TCID50 per nine hours.
http://www.ncbi.nlm.nih.gov/pubmed/0000
3. When in the blood is HIV cell-associated virus, and cell –free virus ?
Asi it is said in experiment that cell – free virus could live nonger in dry blood spot as cell associated virus.
4. What is more infective to body cell free virus or cell associated virus ?
I don’t know if i found it correctly – but here is article
HIV-1-infected lymphocytes can transmit infection across the mucosa more efficiently than cell-free virus.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC0000/
5. As you said that for other exposer not injection of dirty hiv blood in to blood stream as one of the highest risk , other have much smaller risk is it because immunity will fight the virus, but when it is injected deep in blood stream for immune cells it is harder to avoid infection is it so ?
6. If i practice free style wrestling and i have small scratch on my skin I m at small risk of developing hiv infection.
It could 0.1 – 0, 006 % as it is said in one material, it is hard to name one number but risk is rather low. Do you agree ?
http://www.pkids.org/files/pdf/phr/08-02kidsports.pdf
Brief Answer:
Re: HIV transmission
Detailed Answer:
Hello,
Welcome back and thanks for your further follow up.
Regarding your further queries:
1. there is no risk in such kind condition. Mosquito bites are very minute bites and never results in any entry point of HIV virus and there is no broken in skin in that case.
2. I beg your pardon for this part of query as I am clinician so is better to be dealt by a pathologist or microbiologist as I deal with clinical aspect more.
3. Once HIV virus enter into the body it basically targets CD 4 helper cells. Once it is associated with the same it produces further progenies which further infects the cells. The cell free virus dies faster than the one which is associated with cells.
4. Both cell free virus and cell associated virus are invective but cell associated viruses are more infective.
5. Deep invasion of viruses are always more likely to cause infection as compared to superficial invasion.
6. It is very unlikely to get virus invasion through a scratch after free style wrestling. I have clinically not seen or observed such kind of incidence in my clinical experience.
I hope above information helps.
With regards ,
Dr Sanjay Kanodia
MD Dermatology & Sexually transmitted diseases
Re: HIV transmission
Detailed Answer:
Hello,
Welcome back and thanks for your further follow up.
Regarding your further queries:
1. there is no risk in such kind condition. Mosquito bites are very minute bites and never results in any entry point of HIV virus and there is no broken in skin in that case.
2. I beg your pardon for this part of query as I am clinician so is better to be dealt by a pathologist or microbiologist as I deal with clinical aspect more.
3. Once HIV virus enter into the body it basically targets CD 4 helper cells. Once it is associated with the same it produces further progenies which further infects the cells. The cell free virus dies faster than the one which is associated with cells.
4. Both cell free virus and cell associated virus are invective but cell associated viruses are more infective.
5. Deep invasion of viruses are always more likely to cause infection as compared to superficial invasion.
6. It is very unlikely to get virus invasion through a scratch after free style wrestling. I have clinically not seen or observed such kind of incidence in my clinical experience.
I hope above information helps.
With regards ,
Dr Sanjay Kanodia
MD Dermatology & Sexually transmitted diseases
Above answer was peer-reviewed by :
Dr. Chakravarthy Mazumdar
Thank you for your help very much - just some things more !
1. why deep injuries is more ineffective - it is connected with immunity that in skin infection blood stream is slower and an immune cells will better neutralize virus ? Or other factors ?
2. Dry blood is harder to transmit because it densest flow in blood stream if its wound and dry blood contact. is it so ?
3. As we are talking about virus participial that can infect is it virion or RNA particles ? As i understand it should be RNA! is it so ?
Than you !!
1. why deep injuries is more ineffective - it is connected with immunity that in skin infection blood stream is slower and an immune cells will better neutralize virus ? Or other factors ?
2. Dry blood is harder to transmit because it densest flow in blood stream if its wound and dry blood contact. is it so ?
3. As we are talking about virus participial that can infect is it virion or RNA particles ? As i understand it should be RNA! is it so ?
Than you !!
Brief Answer:
Re: HIV transmission and infectivity
Detailed Answer:
Hello,
Welcome back and thanks for your further follow up.
Regarding your further queries:
1. The basic fact in HIV virus is that there is no direct helpful immunity towards the virus. The antibodies which are formed against the virus are not able to fight with the infection in HIV. So in case whether the infection is superficial or deep if the virus enter into body then there are chances of further progression in both conditions.
But in deep invasion of virus the virus directly goes into blood stream and able to cause direct infection.
2. HIV virus is a fragile virus and gets inactivated in dry blood or the interactivity dips down. It is not because of the dense flow of dry blood but declined infectivity of the virus rather than the flow.
3. HIV virus is a single stranded RNA virus with multiple of proteins enveloping it. If there is any change in the structure or its protein then the infectivity declines. So it is the complete virus which is bale to cause the infection and not the RNA alone.
I hope above information helps.
With regards ,
Dr Sanjay Kanodia
MD Dermatology & Sexually transmitted diseases
Re: HIV transmission and infectivity
Detailed Answer:
Hello,
Welcome back and thanks for your further follow up.
Regarding your further queries:
1. The basic fact in HIV virus is that there is no direct helpful immunity towards the virus. The antibodies which are formed against the virus are not able to fight with the infection in HIV. So in case whether the infection is superficial or deep if the virus enter into body then there are chances of further progression in both conditions.
But in deep invasion of virus the virus directly goes into blood stream and able to cause direct infection.
2. HIV virus is a fragile virus and gets inactivated in dry blood or the interactivity dips down. It is not because of the dense flow of dry blood but declined infectivity of the virus rather than the flow.
3. HIV virus is a single stranded RNA virus with multiple of proteins enveloping it. If there is any change in the structure or its protein then the infectivity declines. So it is the complete virus which is bale to cause the infection and not the RNA alone.
I hope above information helps.
With regards ,
Dr Sanjay Kanodia
MD Dermatology & Sexually transmitted diseases
Note: For further follow up on related General & Family Physician Click here.
Above answer was peer-reviewed by :
Dr. Chakravarthy Mazumdar
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