Sign-in to Ask A Doctor - 24x7
OR
Sign in with GoogleGet your health question answered instantly from our pool of 18000+ doctors from over 80 specialties
Western Blot Shows Presence Of GP And P25. ELISA Test And Proviral DNA Qualitative Detection By PCR Were Negative. Do I Have HIV?
hello doctor, good afternoon. i have been quite concerned about a western blot indeterminate result that i got aprox. 6 months ago. with presence of GP160/120 AND p24 bands..
before that exam all my elisa tests where negative.. after that. i did several test's to confirm this result in which are included..
2 elisa tests aprox. 2 months before that indeterminate W/B , i did them in different laboratories. result: NEGATIVE BOTH
1 W/B ( of course i needed to repeat it) , After aprox 3 months of the first W/B , ( aprox. 1 month after elisa´s) result: Negative: NO bands reactive.
And the last the that i did, that was aprox. 1 month ago was a HIV-1 Proviral DNA Qualitative Detection by PCR with a NEGATIVE. result. i specifically did this test on this laboratory: WWW.WWWW.WW
i did all this because of that W/B inderminate result, doctor im much calmed with the subsequence negative results, however there's sort of like a " nail" on my mind regarding the indeterminate result, can you please tell me why does this happen, i mean why and indeterminate and then a negativve result? this make test the W/B Like a not so reliably test, and of course the main question doctor. should i do more testing..? im i completly HIV FREE? thanks very much for your support and help.
before that exam all my elisa tests where negative.. after that. i did several test's to confirm this result in which are included..
2 elisa tests aprox. 2 months before that indeterminate W/B , i did them in different laboratories. result: NEGATIVE BOTH
1 W/B ( of course i needed to repeat it) , After aprox 3 months of the first W/B , ( aprox. 1 month after elisa´s) result: Negative: NO bands reactive.
And the last the that i did, that was aprox. 1 month ago was a HIV-1 Proviral DNA Qualitative Detection by PCR with a NEGATIVE. result. i specifically did this test on this laboratory: WWW.WWWW.WW
i did all this because of that W/B inderminate result, doctor im much calmed with the subsequence negative results, however there's sort of like a " nail" on my mind regarding the indeterminate result, can you please tell me why does this happen, i mean why and indeterminate and then a negativve result? this make test the W/B Like a not so reliably test, and of course the main question doctor. should i do more testing..? im i completly HIV FREE? thanks very much for your support and help.
Wed, 11 Sep 2013
Hi,
Welcome to HCM.
2 ELISA and one Western blot negative for HIV. One WB indeterminate.
Recently we had a discussion on line and found such misinterpretations do occur with Western Blot test. So you can ignore that interpretation of Western Blot indeterminate result as baseless. HIV PCR is more superior than all antibody tests as it directly detects the HIV antigen itself. When this is negative after 4 weeks of high risk activity then you need not bother about other tests and ignore the same.
Very rare possibility is that in presence of HIV 2 infection, an ordinary HIV PCR would not have detected. But it is rare possibility.
Go for an HIV rapid test which can detect both HIV 1&2 separately. If it is also negative for HIV2 also, then just forget about HIV. You are HIV free and no further tests are necessary.
DR S.Murugan
Welcome to HCM.
2 ELISA and one Western blot negative for HIV. One WB indeterminate.
Recently we had a discussion on line and found such misinterpretations do occur with Western Blot test. So you can ignore that interpretation of Western Blot indeterminate result as baseless. HIV PCR is more superior than all antibody tests as it directly detects the HIV antigen itself. When this is negative after 4 weeks of high risk activity then you need not bother about other tests and ignore the same.
Very rare possibility is that in presence of HIV 2 infection, an ordinary HIV PCR would not have detected. But it is rare possibility.
Go for an HIV rapid test which can detect both HIV 1&2 separately. If it is also negative for HIV2 also, then just forget about HIV. You are HIV free and no further tests are necessary.
DR S.Murugan
I find this answer helpful
2 users find this helpful
Note: Get personalized answers to your HIV related queries You can choose to talk face to face with your doctor. Book a Video Call now.
Disclaimer: These answers are for your information only and not intended to replace your relationship with your treating physician.
This is a short, free answer.
For a more detailed, immediate answer, try our premium service
[Sample answer]
Related questions you may be interested in
Recent questions on Elisa
Loading Online Doctors....