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Dr. Andrew Rynne
MD
Dr. Andrew Rynne

Family Physician

Exp 50 years

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Are Findings Showing A Mixed Population Of T- And Polyclonal B-lymphocytes A Cause For Concern?

What does this mean The lymphocyte gate consists of small, hypogranular, CD45 positive cells, which comprise 4% of the total events. The majority are T-lymphocytes (CD3,CD5,CD7) with a normal CD4:CD8 ratio of 2.1. B-lymphocytes (CD19) with a normal kappa:lambda ratio of 1.2 are 4% of the gated population (0.2% of the total cells). No increase in B-cells co-expressing CD5 or CD10 is noted. The blast gate consists of intermediate to large, CD45 dim hypogranular cells. Of these, 0.3% of the total cells are myeloid blasts positive for CD117, myeloid antigen CD33, and hematopoietic progenitor cell antigen (CD34). They are negative for T-lymphocyte markers (CD3,CD5,CD7), B-lymphocyte markers (CD19, kappa, lambda), and CD10. Additionally, a small population of CD10/CD19 positive cells (0.5% of the total events) is seen, in keeping with hematogones. These findings show a mixed population of T- and polyclonal B-lymphocytes with no monoclonal B-lymphoid population identified. There is no increase in blasts and no atypical antigen expression is noted on the myeloid cells.
Tue, 11 Dec 2018
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Pathologist and Microbiologist 's  Response
Hello and Welcome to ‘Ask A Doctor’ service.
I have reviewed your query and here is my advice.

The report which you are talking about is flow cytometry, which is usually done on either peripheral blood or bone marrow.

I think flowcytometry was done for checking remission status of previous leukemia. The report is saying you (the patient) are free from leukemia or you have acquired remission.

Strictly speaking, the report has to be correlated with clinical history. Is there any history of leukemia? What is the history that made you go for this flowcytometry assay.

Hope I have answered your query. Let me know if I can assist you further.

Regards,
Dr. Siddartha
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Are Findings Showing A Mixed Population Of T- And Polyclonal B-lymphocytes A Cause For Concern?

Hello and Welcome to ‘Ask A Doctor’ service. I have reviewed your query and here is my advice. The report which you are talking about is flow cytometry, which is usually done on either peripheral blood or bone marrow. I think flowcytometry was done for checking remission status of previous leukemia. The report is saying you (the patient) are free from leukemia or you have acquired remission. Strictly speaking, the report has to be correlated with clinical history. Is there any history of leukemia? What is the history that made you go for this flowcytometry assay. Hope I have answered your query. Let me know if I can assist you further. Regards, Dr. Siddartha