The question you have is how long can Clostridium difficile spores remain in the environment and the answer is a long time. Most spores are resistant to topical treatment except for diluted bleach. I would recommend cleaning all surfaces in and around the bathroom than may have come in contact with feces. I also recommend frequent and good hand washing techniques after using the bathroom and even visiting people in hospitals or nursing homes. A common medication over the counter called omeprazole or PPIs may contribute to Clostridium difficile colitis and I recommend stopping this if you are taking it. I hope this answers your question. if you have any further questions please contact us again.