Hello Pankaj! Trigaine is Minoxidil solution that comes in various strengths of 2, 5 &10% and is used as anti hair fall topical therapy. Usually 1ml twice daily application is suggested. I usually prescribe it for patients of androgenic alopecia along with Finasteride and multivitamin-mineral tablets. Shampooing frequency while using minoxidil should be once daily. I suggest my patients to keep their hair short, wash daily with mild shampoo, use conditioner and let the hair dry. Once the hairs are dry apply 1ml minoxidil carefully on the scalp using the applicator in thinning area. Style as desired and go to work. In the evening/night apply 1 ml without wash/shampoo. If you want to use oil, apply it 2 hours AFTER minoxidil application. Repeat every day. Hope you found this answer helpful. Feel free to ask more.