Name : Mrs. ANJANA KUMARI-0000 Collected : 14/10/2013 12:21:00PM Lab No. : 0000 Age: 32 Years Gender: Female Received : 14/10/2013 2:48:26PM Reported : 18/10/2013 10:00:08PM A/c Status Test Name : P Ref By : DR. G.C. SINGHAL Results Report Status : Final Units Ref. Range PML-RARA GENE REARRANGEMENT, PCR QUALITATIVE (Real Time PCR) Note Positive 1. Sensitivity of the assay is 0.01% when copies of ABL detected is 100,000 2. Limit of detection is 10 copies of PML-RARA fusion gene transcripts per PCR 3. This is an in-house developed assay designed as per EAC (Europe Against Cancer) protocol 4. This test detects 3 types of translocations - bcr 1, bcr 2 & bcr 3 5. Test conducted on Whole blood / Bone Marrow Comments Acute Promyelocytic Leukemia (APL) is characterized by a unique reciprocal chromosomal translocation t(15;17) (q22;q11-12) and its underlying fusion gene PML / RARA rearrangement. The fusion is seen between Promyleocytic (PML) gene on chromosome 15 and RARA gene on chromosome 17. Based on PML breakpoint location, the PML RARA transcripts subtype bcr 1 & bcr 2 (Long transcript type) and bcr 3 (Short transcript type) may be formed. Uses • For diagnostic identification of PML RARA in cases of Acute Promyelocytic Leukemia • To assess molecular resistance & predict response to treatments containing ATRA and / or ATO Dr.Atul Thatai PhD ( Biotechnology ) HOD Molecular Diagnostics -------------------------------End of report --------------------------------